->benny - Vista 32bit still has NTVDM so it can run some 16bit stuff, but some bits like int 10h for changing screen mode seem to have been removed...64bit Vista has had all that clutter removed, and about time.
Back to this though - how does a .com end up running a 32bit Windows program? Some serious EXE hacking going on there! For instance none of the Flashtro programs will work.
What packer are you using Optimus?
Jim